Development Of Different Forms Research Articles

The advancement of echocardiography through evolutionary development

Echocardiography, a non-invasive diagnostic method that offers insights into cardiac structure, function, and hemodynamics, has emerged as a key component of cardiovascular medicine. In a span of less than five decades, this technique has undergone significant evolution, becoming one of the most commonly utilized cardiovascular diagnostic tests after electrocardiography. This article provides a concise overview of the evolutionary development of different forms of echocardiography.

The deployment of advanced practice nurses in the French health system: From clinics to professional networks.

The aim of this study is to contribute to an understanding of the role deployment of advanced practice nurses (APNs) in French healthcare settings. The introduction of APNs was formalised in France by the decrees issued on 18 July 2018, which described the areas, activities and training of APNs. A qualitative study on the role implementation of APNs was conducted between July 2021 and May 2022 following a call for projects launched by the Île-de-France Regional Health Agency to evaluate the deployment of APNs in the area. Data were collected through field observations and semi-structured interviews in order to explore both the APNs deployment processes in nine healthcare structures and the roles played by APN networks and associations with regard to the deployment of APN activities in their working environments. The projects proved to be evolutionary, and their development was marked by various forms of APN isolation and multiple obstacles that were specific to their professional practice settings. Some APNs relied on a variety of forms of mutual assistance and advocacy deployed throughout APN networks and associations. The deployment of APNs' role was impacted by diverse configurations of professional power relations and the nature of the obstacles that were structural for APNs in primary care. Their experience of isolation derived from the novelty of their role, the challenge they posed to the cohesion of the nursing profession and a lack of supportive policies for their deployment. Their participation in APN networks and associations enabled them to access advocacy and manage the uncertainties and unknowns related to the deployment of their activities. The results suggest that the formalisation of schemes for mutual assistance among APNs and advocacy should be integrated into the guidelines for the implementation of their role. APN policy should strengthen a bottom-up approach, relying in particular on the development of different forms of collaboration and communication between APN networks and associations on the one hand and the public authorities on the other.

Smoking cessation strategies: An in-depth analysis of nicotine replacement therapy and alternatives

Introduction and purpose: The primary objective of this literature review is to thoroughly investigate the topic of nicotine replacement therapy, considering the latest discoveries and detailing various forms of available nicotine substitution. Materials and methods: Literature review of PubMed and Google Scholar databases. State of Knowledge: Tobacco smoking remains one of the leading causes of premature death worldwide, accounting for millions of deaths each year. Nicotine, the addictive component of tobacco, is responsible for sustaining cigarette smoking patterns and making smoking cessation particularly challenging. Nicotine replacement therapy (NRT) is now considered one of the primary methods to help individuals quit smoking by reducing withdrawal symptoms and cravings associated with cessation. Conclusions: Over the years, extensive research has confirmed the effectiveness and safety of NRT, resulting in the development of different forms of nicotine substitution. The outcomes of using these various forms are similar and premature discontinuation by patients remains a primary reason for NRT failure. Therefore, it is crucial for each patient to select the form of NRT that meets their individual preferences. The best results are obtained by combining multiple forms of substitution simultaneously, as well as integrating them with other therapeutic methods.

Political parties, interest groups and the contestations of home care: the case of France

This article makes a contribution to the literature on the new politics of the welfare state. Taking the case of home care for the elderly in France, it shows that contestation around this policy has been rooted more in the opposition between different interest groups as regards marketisation than in gender issues. However, in a highly feminised sector, the results of this contestation have had gendered effects. Neither left- nor right-wing governments have sought to halt the process of marketisation; instead, they have replicated the opposition between interest groups and defended the development of different forms of care marketisation, with different, gendered consequences for the valuation of care work.

Humanness as performance

AbstractIf archaeology is the examination of historical conditions with reference to a surviving material residue, then one way in which these conditions might be characterized is as the different ways they had enabled the development of different forms of humanness. The historical construction of this diversity is discussed here as the ways that the relationships between humans and things had been performed. This means that the practice of archaeology must question the recent desire to adopt a flat ontology that defines archaeology as the ‘discipline of things’. It is argued that it was by means of the performances established between humans and their various objects of concern that different forms of human life were able to define themselves. The implications of this argument for the practice of archaeology are explored.

Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.

Cancer pagurus is highly susceptible to shell disease syndrome. However, little is known about concomitant changes in the epibacterial community. We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA. Within each spot, bacterial communities of affected areas were less diverse compared to communities from nonaffected areas. Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas. This indicates a reduced and shifted microbial community composition caused by the black spot disease. Different communities found in black spots likely indicate different stages of the disease. In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome. We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing. The ability to degrade chitin was mainly found for Gammaproteobacteria and Aquimarina spp. within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria. One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations. The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus. IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species. Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas. Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell. By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases. Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease. Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.

Resilience and psychosocial functioning in schizotypy.

Understanding resilience has expanded our knowledge of certain risk and protective factors regarding the development of different forms of psychopathology. Particularly, a focus on resilience can be used to implement interventions and to target specific behaviors in hopes of mitigating the onset of a disorder or to alleviate symptoms. Less research on resilience has been done with individuals with psychosis, particularly schizophrenia spectrum disorders. Only 1 previous study has targeted individuals assessed for schizotypy. The current study examined associations between resilience and schizotypy features, assessed using self-report measures. Specifically, we compared 3 different resilience measures, social and occupational functioning, and 3 schizotypy measures in 3 different samples: undergraduate students (n = 878), adult community members (n = 120), and an Amazon Mechanical Turk adult community members (n = 329). Data analyses consisted of correlation and regression analyses, including tests for statistical moderation. Specifically, this study found negative associations between schizotypy and both psychosocial functioning and resilience. Although we predicted resilience would moderate the relations between schizotypy and psychosocial functioning, our analyses did not provide support for a moderating role for resilience. We discuss our findings in terms of understanding the relations among schizotypy, resilience, and psychosocial outcome constructs. (PsycInfo Database Record (c) 2022 APA, all rights reserved).

The role of neutrophil extracellular traps in acute lung injury.

Acute lung injury (ALI) is a heterogeneous inflammatory condition associated with high morbidity and mortality. Neutrophils play a key role in the development of different forms of ALI, and the release of neutrophil extracellular traps (NETs) is emerging as a common pathogenic mechanism. NETs are essential in controlling pathogens, and their defective release or increased degradation leads to a higher risk of infection. However, NETs also contain several pro-inflammatory and cytotoxic molecules than can exacerbate thromboinflammation and lung tissue injury. To reduce NET-mediated lung damage and inflammation, DNase is frequently used in preclinical models of ALI due to its capability of digesting NET DNA scaffold. Moreover, recent advances in neutrophil biology led to the development of selective NET inhibitors, which also appear to reduce ALI in experimental models. Here we provide an overview of the role of NETs in different forms of ALI discussing existing gaps in our knowledge and novel therapeutic approaches to modulate their impact on lung injury.

The Role of microRNA in the Development, Diagnosis, and Treatment of Cardiovascular Disease: Recent Developments.

Since their discovery in 1993, microRNAs (miRNAs) have emerged as important regulators of many crucial cellular processes, and their dysregulation have been shown to contribute to multiple pathologic conditions, including cardiovascular disease (CVD). miRNAs have been found to regulate the expression of various genes involved in cardiac development and function and in the development and progression of CVD. Many miRNAs are master regulators fine-tuning the expression of multiple, often interrelated, genes involved in inflammation, apoptosis, fibrosis, senescence, and other processes crucial for the development of different forms of CVD. This article presents a review of recent developments in our understanding of the role of miRNAs in the development of CVD and surveys their potential applicability as therapeutic targets and biomarkers to facilitate CVD diagnosis, prognosis, and treatment. There are currently multiple potential miRNA-based therapeutic agents in different stages of development, which can be grouped into two classes: miRNA mimics (replicating the sequence and activity of their corresponding miRNAs) and antagomiRs (antisense inhibitors of specific miRNAs). However, in spite of promising preliminary data and our ever-increasing knowledge about the mechanisms of action of specific miRNAs, miRNA-based therapeutics and biomarkers have yet to be approved for clinical applications. SIGNIFICANCE STATEMENT: Over the last few years microRNAs have emerged as crucial, specific regulators of the cardiovascular system and in the development of cardiovascular disease, by posttranscriptional regulation of their target genes. The minireview presents the most recent developments in this area of research, including the progress in diagnostic and therapeutic applications of microRNAs. microRNAs seem very promising candidates for biomarkers and therapeutic agents, although some challenges, such as efficient delivery and unwanted effects, need to be resolved.

Sustained Depolarization Induces Homeostatic Plasticity in Mouse and Human Sensory Neurons

Remodeling of the nervous system serves as a protective mechanism during abrupt disturbance of neuronal homeostasis. This offers adequate maintenance of overall network activity that includes both synaptic scaling and regulation of intrinsic neuronal excitability in an adaptive process known as homeostatic plasticity. Although alterations in neuronal excitability of nociceptors have been associated with the development of different forms of chronic pain, the involvement of homeostatic plasticity in the excitability of nociceptors, in the context of prolonged excitation, and in the pathophysiology of chronic pain is poorly understood. Using a combination of pharmacological, optogenetic and electrophysiological approaches, we show that sustained depolarization of cultured dorsal root ganglia (DRG) neurons, especially nociceptors, strongly reduces intrinsic excitability of mouse and human DRG neurons. These changes include decreases in input resistance, action potential (AP) fall time and AP half width, and an increase in rheobase associated with an overall decrease in AP firing, thus suggesting that mechanisms of homeostatic plasticity are engaged in mouse and human nociceptors. Unexpectedly, RNA sequencing studies show that there are not significant changes in the global mouse and human DRG neuronal gene expression after sustained depolarization, suggesting that transcriptional changes are not the underlying cause of homeostatic plasticity in nociceptors. Our data reveal the presence of intrinsic homeostatic mechanisms that regulate neuronal excitability in response to sustained depolarization of mouse and human nociceptors. Therefore, dysregulation of these homeostatic mechanisms could contribute to the understanding of the pathophysiology of chronic pain syndromes. Grant support from NS042595; The Dr. Seymour and Rose T. Brown Professorship in Anesthesiology K00NS113422 American Neuromuscular Foundation Career Development Award F32DA051160-01.

Insights from genomic studies on the role of sex steroids in the aetiology of endometriosis.

Endometriosis is a chronic neuro-inflammatory disorder the defining feature of which is the growth of tissue (lesions) that resembles the endometrium outside the uterus. Estimates of prevalence quote rates of ~10% of women of reproductive age, equating to at least 190 million women world-wide. Genetic, hormonal and immunological factors have all been proposed as contributing to risk factors associated with the development of lesions. Twin studies report the heritable component of endometriosis as ~50%. Genome-wide association studies (GWAS) have identified single nucleotide polymorphisms (SNPs) that appear over-represented in patients with endometriosis, particularly those with more extensive disease (stage III/IV). In different sample populations, there has been replication of SNPs near genes involved in oestrogen and other steroid regulated pathways including ESR1 (oestrogen receptor alpha), GREB1, HOXA10, WNT4 and MAPK kinase signalling. Comparisons with GWAS conducted on other patient cohorts have found links with reproductive traits (age at menarche) and disorders (fibroids, endometrial and ovarian cancer) and common co-morbidities (migraine, depression, asthma). In summary, genetic analyses have provided new insights into the hormone-regulated pathways that may contribute to increased risk of developing endometriosis some of which may act in early life. New studies are needed to clarify the relationship between the many SNPs identified, the genes that they regulate and their contribution(s) to development of different forms of endometriosis. We hope that more advanced methods allowing integration between GWAS, epigenetic and tissue expression data will improve risk analysis and reduce diagnositic delay.Lay summaryEndometriosis is a debilitating reproductive disorder affecting ~10% of reproductive-age women, and those assigned female at birth, which causes a range of symptoms including chronic pain and infertility. The reason why some, but not all these individuals, develop the lesions that characterise the disease are poorly understood, but recently attention has focused on genetic risk factors to explain why the incidence is higher in some families. Studies on large cohorts of patients with comparison of their DNA to women without endometriosis or with other disorders have documented changes in genes associated with steroid hormone production or action. The results provide further evidence that endometriosis shares genetic risk factors with other disorders of the reproductive system and a platform for new ideas related to risk, biomarkers and therapies.

Role of the SARS-CoV-2 virus in the appearance of new onset type 1 diabetes mellitus in children in Gran Canaria, Spain.

It has been hypothesized that SARS-CoV-2 may play a role in the development of different forms of diabetes mellitus (DM). The Canary Islands have the highest incidence of type 1 DM (T1DM) reported in Spain(30-35/100,000 children under 14 years/year). In 2020-2021 we observed the highest incidence so far on the island of Gran Canaria, as a result of which we decided to evaluate the possible role of COVID-19 in the increased number of onsets. We examined the presence of IgG antibodies against SARS-CoV-2 in children with new onset T1DM between October 2020 and August 2021. We compared recent T1DM incidence with that of the previous 10 years. Forty-two patients were diagnosed with T1DM (48.1/100,000 patients/year), representing a nonsignificant 25.7% increase from the expected incidence. Of the 33patients who consented to the study, 32 presented negative IgG values, with only one patient reflecting undiagnosed past infection. Forty-four percent of patients presented with ketoacidosis at onset, which was similar to previous years. We conclude that there is no direct relationship between the increased incidence of T1DM and SARS-CoV-2 in the region. The COVID-19 pandemic did not result in an increased severity of T1DM presentation.

Insulin resistance in diabetes: The promise of using induced pluripotent stem cell technology.

Insulin resistance (IR) is associated with several metabolic disorders, including type 2 diabetes (T2D). The development of IR in insulin target tissues involves genetic and acquired factors. Persons at genetic risk for T2D tend to develop IR several years before glucose intolerance. Several rodent models for both IR and T2D are being used to study the disease pathogenesis; however, these models cannot recapitulate all the aspects of this complex disorder as seen in each individual. Human pluripotent stem cells (hPSCs) can overcome the hurdles faced with the classical mouse models for studying IR. Human induced pluripotent stem cells (hiPSCs) can be generated from the somatic cells of the patients without the need to destroy a human embryo. Therefore, patient-specific hiPSCs can generate cells genetically identical to IR individuals, which can help in distinguishing between genetic and acquired defects in insulin sensitivity. Combining the technologies of genome editing and hiPSCs may provide important information about the genetic factors underlying the development of different forms of IR. Further studies are required to fill the gaps in understanding the pathogenesis of IR and diabetes. In this review, we summarize the factors involved in the development of IR in the insulin-target tissues leading to diabetes. Also, we highlight the use of hPSCs to understand the mechanisms underlying the development of IR.

Strategic symbiotic arrangements: how can contract breweries help change a local market into part of an integrated broader market

The impacts of the craft beer revolution reached Brazilian beer industry in the first years of this century. The evolution of the craft beer segment is paving the way to the development of different forms of organising in Brazil, such as contract brewing, designed for firms that do not own physical brewing facilities. This form of organising has several combinations, which vary according to the firm's value proposition. Contract brewing may be a reflection of open strategy practices, which affect both organisational structures and ownership forms. This research on the contract brewing business model attempts to advance our understanding of strategic symbiotic arrangements in this particular industry. After examining how contract breweries are operating in practice, we were able to group the business model's key components into two different configurations - symbiotic and transitional, whose main challenges are closely related to their interdependencies with a production partner and their interorganisational strategy.

Role of microRNA in pancreatic beta cell function.

MicroRNA (miRNAs) are small non-coding RNA involved in gene expression regulation. Emerging evidences identify miRNAs as key regulators of beta cell physiology. Their role in fine-tuned gene expression regulation is crucial in the differentiation of insulin-producing cells and contributes to the acquisition and management of their unique phenotype. Dysregulation of miRNA expression causes beta cell dysfunction and promotes the development of different forms of diabetes mellitus.

Development of inter-municipal cooperation as a factor in the intensification of investment processes in a region (on the example of the Vologda region)

Currently, the heterogeneity of economic space, relatively low development rates and negative trends in the socio-economic development of individual territories, and limited resource opportunities (including investment opportunities) are among the significant problems of municipalities. One of the mechanisms for solving them at the level of local government bodies is the institution of inter-municipal cooperation. The aim of the paper is to analyze the possibilities of territorial and sectoral inter-municipal cooperation aimed at overcoming the negative imbalances of the region's investment development (based on a case study of the Vologda region). The methods of cluster analysis and time series analysis were used, as well as tabular and graphic methods for the visual representation of the study results, the application packages MS Excel and STATISTICA. The paper examines the main trends in the development of inter-municipal cooperation processes in the Russian economy, identifies the specifics of the interaction of municipal entities in the Vologda region, and presents a territorial and sectoral approach to the development of different forms of cooperation based on the example of the Vologda region. The obtained results can be used by regional and local authorities in the development of investment policy, the formation of a system of organizational and economic mechanisms for the intensification of investment processes in the region. The study materials presented in the paper may be interesting for scientific discussion, for use in scientific and educational activities.

Why are they Committed? Examining the Entrepreneurial Process with Side-Bet Theory

Along with an emerging body of literature investigating the volitional phase of the entrepreneurial process, the present research examines the determinants of entrepreneurial commitment, using Becker’s (1960) side-bets theory. Originally developed in the organizational setting, side-bet theory is a relevant framework to understand what compels individuals into consistent courses of actions. Powell and Meyer (2004) proposed seven category of side-bets and showed that they explained the development of different forms of commitment among employees, relying on Allen and Meyer (1990) multidimensional model of commitment. In this research, we propose to apply this seven-category lens to the context of emerging organizations, to further understand the development nascent entrepreneurs’ commitment profiles. After a pilot study designed to adapt Powell and al (2004)’s categorization to entrepreneurial activity, we operationalized it in a quantitative study (n=177) on nascent entrepreneurs. We examined how they relate and influence Meyer and al (2006)’s two basis of commitment – value-based and exchange-based -, targeting two foci relevant to the entrepreneurial process: entrepreneurial project and profession. Our results show that each form of nascent entrepreneur’s commitment is driven by a unique combination of side-bets. Therefore, it contributes to a better understand of nascent entrepreneurs’ mindset during the volitional phase of the entrepreneurial process.

From Representative to Anti-Civic Populist Democracy?

This article analyses the emergence and development of different forms of civil society in Bulgaria from the late 1980s to the present day, focusing on ngos and the large anti-government protests in 1989–1991, 1997, and 2013–2014. It shows that civil society has been developing in ebbs and flows, its main actors having alt-civic and fake doubles: nationalist movements and fake counter-protests. Recent developments indicate a clear trend of transition from representative to direct democracy, which coincides with the populist orientation of most parties. This coincidence is dangerous because populist parties, following the romantic tradition, reinvented the figure of “the people” as traditionalistic, nationalistic, and conservative. “Civil society,” seen as “alien,” was constructed as an enemy of “the people.” The author argues that defending the pluralistic values of civil society against the thus-invented “people,” is the main challenge to democracy in Bulgaria today.

A Modeling Study of the Emergence of Eye Position Gain Fields Modulating the Responses of Visual Neurons in the Brain.

The responses of many cortical neurons to visual stimuli are modulated by the position of the eye. This form of gain modulation by eye position does not change the retinotopic selectivity of the responses, but only changes the amplitude of the responses. Particularly in the case of cortical responses, this form of eye position gain modulation has been observed to be multiplicative. Multiplicative gain modulated responses are crucial to encode information that is relevant to high-level visual functions, such as stable spatial awareness, eye movement planning, visual-motor behaviors, and coordinate transformation. Here we first present a hardwired model of different functional forms of gain modulation, including peaked and monotonic modulation by eye position. We use a biologically realistic Gaussian function to model the influence of the position of the eye on the internal activation of visual neurons. Next we show how different functional forms of gain modulation by eye position may develop in a self-organizing neural network model of visual neurons. A further contribution of our work is the investigation of the influence of the width of the eye position tuning curve on the development of a variety of forms of eye position gain modulation. Our simulation results show how the width of the eye position tuning curve affects the development of different forms of gain modulation of visual responses by the position of the eye.

Proteomic Analysis of Myocardia Containing the Obscurin R4344Q Mutation Linked to Hypertrophic Cardiomyopathy.

Obscurin is a giant cytoskeletal protein with structural and regulatory roles encoded by the OBSCN gene. Recently, mutations in OBSCN were associated with the development of different forms of cardiomyopathies, including hypertrophic cardiomyopathy (HCM). We previously reported that homozygous mice carrying the HCM-linked R4344Q obscurin mutation develop arrhythmia by 1-year of age under sedentary conditions characterized by increased heart rate, frequent incidents of premature ventricular contractions, and episodes of spontaneous ventricular tachycardia. In an effort to delineate the molecular mechanisms that contribute to the observed arrhythmic phenotype, we subjected protein lysates prepared from left ventricles of 1-year old R4344Q and wild-type mice to comparative proteomics analysis using tandem mass spectrometry; raw data are available via ProteomeXchange with identifier PXD017314. We found that the expression levels of proteins involved in cardiac function and disease, cytoskeletal organization, electropotential regulation, molecular transport and metabolism were significantly altered. Moreover, phospho-proteomic evaluation revealed changes in the phosphorylation profile of Ca2+ cycling proteins, including sAnk1.5, a major binding partner of obscurin localized in the sarcoplasmic reticulum; notably, this is the first report indicating that sAnk1 undergoes phosphorylation. Taken together, our findings implicate obscurin in diverse cellular processes within the myocardium, which is consistent with its multiple binding partners, localization in different subcellular compartments, and disease association.